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Organoids: from traditional 2D cell Culture to 3D culture models
In recent years, organoid culture technology is developing rapidly. Organoids has been awarded 'Method of the Year 2017' by Nature Methods, for their immense potential as tools to study human biology in health and disease.
The ‘organoid’ was defined by Fatehullah et al. As an in vitro 3D cellular cluster derived exclusively from primary tissue, ESCs or iPSCs, capable of self-renewal and self-organization, and exhibiting similar organ function as the tissue of origin.
Recently, organoids have been widely used in many areas, including developmental biology, disease modeling, precision medicine, regenerative medicine, toxicology, drug discovery studies, host-microbiome interactions, gene editing, multi-omics, and phylogenetic studies.
An organoid is a 3D multicellular in vitro tissue construct that mimics the complex structure and functionality of corresponding in vivo organ.
Organoids recapitulate multiple biological parameters including the spatial organization of heterogeneous tissue-specific cells, cell-cell interactions, and cell-matrix interactions, and certain physiological functions generated by tissue-specific cells within the organoid.
Organoids are typically cultured in an extracellular matrix (ECM) surrounded by culture media supplemented with niche factors specific to the organoid type. Common niche and ECM factors: R-spondin (RSPO1), EGF, Noggin, Activin A, Collagen.
Explore the MedChem Express catalog of organoid related products:
Biochemical assay reagents and Natural products
Click here to download the "Organoid Culture" handbook
ACROBiosystems, dedicated to advancing drug development by delivering innovative solutions, has newly launched the Organoid Toolbox. This comprehensive toolbox includes ready-to-use organoids, organoid differentiation kits, as well as diverse services, including organoid development service, organoid disease model development service and organoid testing service, etc., so as to accelerate the process of your drug development projects.
A Wide Range of Organoid Solutions
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AcroBiosystem offers also more related tools:
Mogengel Matrix for Organoid Culture
Cytokines for Organoid Culture
Request a free sample of Mogengel Matrix
HumanKine® Growth Factors are the perfect tool for your cerebral organoid research
Cerebral organoids are a powerful tool to study neurodevelopment and implementing drug screening for neurological disorders. These structures are generated from induced or human pluripotent stem cells (iPSCs, hPSCs) grown in specialized media with growth factors.
HumanKine® recombinant cytokines and growth factors are authentic human proteins that are HEK293 cell expressed, and demonstrate greater activity than E. coli-expressed proteins. They are also tag and endotoxin-free.
Growth factors and cytokines for organoid research
Qkine provides a complete portfolio of animal-free and carrier protein-free growth factors and cytokines for organoid culture.
Click here to download the "Growth factors for organoids" brochure
Growth factors for organoid culture media
 Liver Organoid |
 Lung Organoid |
 Intestinal Organoid |
 Cardiac Organoid |
 Brain Organoid |
 Breast organoid |
Watch the video!
3D Cell Culture: Assay Guide
Three-dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion.
3D cell culture more closely resembles physiological conditions and can provide a better indicator of success in vivo. Assays and kits exist which can help cultivate and quantify 3D cell cultures.
AssayGenie offers tools for cultivation and 3D cell culture assays:
3D Organoid Cell Viability Assay
high-throughput LDH cytotoxicity assay
XTT Cell Proliferation Assay Kit
3D cell culture & Extracellular matrices
Cells that form tissues and organs are situated within a complex 3D structure known as the extracellular matrix (ECM). Despite this, a high proportion of the current research being undertaken on these cells utilizes 2D cultures. The shape, size, and functionality of a cell is mostly affected by the physical environment in which it is grown, therefore 3D cell cultures have proven to provide a more adequate model of in vivo physiological conditions.
AMSBIO provides a wide range of unique 3D cell culturing products including natural ECM, recombinant ECM, scaffolds, hydrogels and spheroid formation solutions.
See all 3D cell culture & Extracellular matrices products
Organoid cell culture & cryopreservation
Organoid Growth Organoid Harvesting Organoid Storage Resources
3D cell culture related products
As a leading provider of 3D cell culture solutions, Creative Bioarray offers a wide range of 3D cell culture related products to support your in-house development of 3D models, including products for spheroids formation, 3D cell culture scaffolds, bioreactors, and 3D cell culture system.
3D models provide microenvironments which can more accurately reflect the natural environment inside of a living organism. Cells embedded in a 3D environment exhibit better intercellular interactions with more physiological responses. With inherent proliferative and metabolic (oxygen, nutrients, and wastes) gradients, 3D cell cultures serve as excellent models which have been extensively employed in cancer biology, tissue engineering, and regenerative medicine and drug discovery.
3D Scaffolds
Polycarprolactone (PCL) Scaffolds
Alginate Scaffolds
Hydrogel Scaffolds
3D Mutiwell Plate
Micropatterned surface plate
Ultra-low attachment plate
Hanging drop plate
3D Bioreactor
3D-Flo Cell Culture System
Purchase all these products through DBA Italia
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