DBA Italia offers custom oligonucleotide and probe synthesis through our partner company Eurogentec. 

Custom oligos

Eurogentec proposes a large choice of chemistries, modifications, specifications and 
purifications. More than 300 modifications and several purity levels are available.

Length: From 5 to 139 bases

Synthesis scale: 10 nmol • 40 nmol • 200 nmol • 1000nmol • 2.5 µmol • 5µmol • 10 µmol

Backbones: Eurogentec synthesises classic DNA and RNA based oligos but other backbones like LNA®, 2’O-Me, 2’O-MOE and PNA can be used to match your specifications.

• DNA
• RNA 
• LNA® (Locked Nucleic Acid) is a bicyclic nucleic acid with a structure locked into a rigid C3'-endo position, which favours RNA A-type helix  duplex geometry. This exceptional structure confers a very strong thermal stability towards complementary DNA and RNA template suitable for  hybridisation assays requiring high specificity and/or reproducibility
•  2’O-Me RNA: are partially resistant to a variety of ribo- and deoxyribonucleases. They form more stable hybrids with complementary RNA strands 
  than equivalent DNA/RNA sequences. They are ideal for antisense probes.
• 2’O-MOE RNA: have an analogue chemical structure to RNA excepted that a methoxy-ethyl residue 
  is attached at the 2’-O-position. The chemical group at this position confers to the oligo backbone a highest nuclease resistance and a better binding  affinity compared to the classical RNA molecule making it a useful tool for antisens applications.
• PNA :  is an artificial DNA/RNA analogue with no charged backbone. The absence of charge repulsion between PNA and the DNA/RNA complementary  strand confers a higher specificity and sensitivity. PNA are also known to be resistant to the enzyme degradation and stable over a wide range of pH,  temperature and salt concentrations. These properties enable PNA to be particularly useful for FISH studies and miRNA inhibition.
• Linkages: Phosphorotioate and Methylphosphonate links

Modifications: More than 300 modifications

Purifications: SePOP desalting, RP-Cartridge•Gold™, HPLC, in vivo, PAGE, Dual HPLC Quality

Control: MALDI-TOF MS

Format: Dried (except for unmodified SePOP desalted oligonucleotides from 15 to 39 DNA bases: 100 µM H2 O by default)

Packaging: 2 mL tube, 96-well or 384-well plates

Documentation: Technical data sheet

Shipping: At room temperature

NGS oligos

Next-Generation Sequencing (NGS) is a high-throughput technology allowing the massive sequencing of nucleic acids following a DNA library preparation. After DNA fragmentation, adapters (including indexes for multiplexing) are fused to the fragments. NGS adapters require both a high level of purity (no n-x side products) and the absence of cross-contamination (confusing index sequences).

Eurogentec can support your sequencing projects by offering dedicated NGS grade oligonucleotides. Specially produced in order to avoid cross contamination they reduce barcode misalignment during multiplex next generation sequencing projects. 

RNAi oligos

RNA interference is a mechanism of gene silencing at the mRNA level. This phenomenon is triggered by small interfering (si)RNAs and micro (mi)RNAs. siRNAs and miRNAs regulate gene expression. They can activate the degradation of the targeted mRNA or prevent its translation.

Eurogentec provides custom synthesis of siRNA oligonucleotides at small and large scales and can assist you for the design of your sequences for free.

Length: From 21 to 27 bases

Delivered quantity: 3 nmol • 7 nmol • 12 nmol • 22 nmol • 40 nmol• 60 nmol • 80 nmol • 200 nmol

Backbone: RNA, LNAR, 2’O-Me RNA, 2'O-MOE RNA and all linkages (see the backbone paragraph in the "Custom Oligos" section)

Modifications: 5’:Phosphate, 6-FAM, CyR3, Cy5R, TET, HEX,...

3’: DABCYL, TEG-Cholesteryl, TAMRA...

Purifications: SePOP Desalting, IEX-RP/HPLC or in vivo

Quality Control: MALDI-TOF MS

Format: Dried

Packaging: 2 mL tube

Documentation: Technical data sheet

siRNA Design: Free and guaranteed

Shipping: At room temperature

qPCR Probes

Eurogentec offers a wide range of fluorophores and quenchers in various combinations to fit any method and Real-Time thermocycler. 

Dye-Quencher compatibility table

Length: From 15 to 50 bases

Synthesis scale: 10 nmol • 40 nmol • 200 nmol• 1000 nmol • 2.5 μmol • 5 μmol • 10 μmol

Backbone: DNA, LNAR, 2’O-Me RNA and phosphodiester linkage (see the backbone paragraph in the "Custom Oligos" section)

Modifications: 5’: 6-FAM, HEX, CyR3, TET , Cy5R…

3’: TAMRA, DABCYL, BHQ™, DDQ…

Purifications: RP-HPLC or Dual HPLC

Quality Control:MALDI-TOF MS and analytical HPLC

Format: Dried or in solution

Packaging: 2 mL tube

Documentation: Technical data sheet

Probe Design: Available on request

Shipping: At room temperature

Double-dye probes: EUROGENTEC OFFERS a large range of fluorescent dyes and quenchers including proprietary efficient molecules: iLyte Fluor™ dyes and QXL™ quenchers.

Molecular beacons: EUROGENTEC IS a licensed supplier of Molecular Beacons and offers standard,  wavelength-shifting and 2' O-Me RNA molecular beacon.

MGB probes: MGB probes perfectly suited for patient management. MGB increases the Tm of a probe because of its minor groove binding ability. MGB probes are more specific, more efficient and more sensitive than standard double-dye probes. EUROGENTEC provides a complete offer with more than 15 dyes covering all qPCR channels.

If interested in one of these products request an official quote.

Additional materials:

Synthesis scale vs guaranteed yield: The synthesis scale refers to the amount of raw material used to start the synthesis of oligonucleotides. The yield corresponds to the amount of final product recovered at the end of the synthesis and purification processes. The length, the sequence, the type/number of modifications and the purification, strongly influence the reaction yield.

Oligo reconstitution, stability, storage